Effect of vancomycin on the growth of psittacosis-trachoma agents cultivated in eggs and cell culture.
نویسندگان
چکیده
The antibiotic vancomycin was effective in preventing bacterial contamination during studies with psittacosis and trachoma (PT) agents. This antibiotic was not toxic to chick embryos at 80 mg per egg, or to HeLa 229 cells cultivated in a medium containing 3,200 mug/ml of vancomycin; however, it was toxic to LLC-MK2 cells at a concentration of 1,600 mug/ml. Vancomycin did not affect the growth of a variety of PT agents at a concentration of 2 mg per egg or 800 mug per ml of cell culture medium, but it did inhibit the growth of common gram-positive bacterial air contaminants.
منابع مشابه
Research Article: In vitro investigation on response of cultivated hepatocytes from Klunzinger\'s mullet, Liza klunzingeri, to temperature and fetal bovine serum
The appropriate temperature and growth supplementary factors such as fetal bovine serum (FBS) are the necessities needed for cell growth in culture medium. In this regards, the effect of Leibovitz-15 (L-15) medium containing different concentrations of fetal bovine serum (FBS) was assessed on growth and proliferation of cultivated liver cells of klunzingerchr('39')s mullet, Liza klunzingeri, in...
متن کاملViral susceptibility range of the fathead minnow (Pimephales promelas) poikilothermic cell line.
The viral susceptibility range of a poikilothermic cell line derived from the fathead minnow (Pimephales promelas) (FHM) to infection by a number of homoiothermic viruses representing most of the presently recognized viral groups and a member of the psittacosis-lymphogranuloma-trachoma group of agents was studied. All infectious agents, except poliovirus types 1 and 3, infectious bursal agent, ...
متن کاملEffect of gentamicin on growth of viral, chlamydial, and rickettsial agents in mice and embryonated eggs.
Gentamicin, at concentrations up to 500 mug/ml, showed no effect on the replication, yield, or infectivity of seven viruses or on the agents of psittacosis and meningopneumonitis when grown in mice or embryonated eggs. At the 500 mug/ml level, lymphogranuloma venereum cultures had a slight reduction in infectivity. Rickettsia akari demonstrated susceptibility at the 5 mug/ml level, whereas R. r...
متن کاملEffect of culture depth on the growth of Gracilariopsis persica with the potential for mariculture in the Persian Gulf (Bandar Abbas)
We conducted this study to investigate the effect of culture depth of red algae Gracilaropsis persica on the growth and biomass production of this species. Three culture depth of 5, 6, and 7 m were used to cultivate algae in the Persian Gulf (Bandar Abbas) for 45 days. Algae was collected from its natural environment and cultivated on polyethylene rope in the sea. We measured relative growth ra...
متن کاملSymposium on trachoma
In 1957 T'ang and associates in Peking isolated a virus from trachoma in the yolk sac of embryonated eggs. By determining that streptomycin but not penicillin could be used they were able to keep the isolated organisms sterile and in passage. Since then, a number of laboratories around the world have been able to isolate trachoma viruses and grow them in the laboratory. Many workers, like mysel...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied microbiology
دوره 15 1 شماره
صفحات -
تاریخ انتشار 1967